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The Live Cell Imaging Facility (LCIF)

Cutting-edge microscopy and image analysis services, available to researchers at UM as well as those in academia and industry

The Live Cell Imaging Facility offers advanced microscopy technology for capturing high-quality images of both live and fixed cells. From experiment design to image analysis, we provide technical support to help you achieve your imaging objectives. Explore our website to learn more about our services and capabilities. Our users investigate a variety of topics including genetic modifiers of neurological conditions, synaptic changes in neurodevelopmental and neurodegenerative disorders, neuron-glia interactions, glia-regulated neurovascular coupling, and brain and spinal cord injury mechanism and repair. To address such questions, our users take advantage of our advanced imaging modalities to assess dendritic length and branching, synaptic counts, synapse component in cell culture and brain slice, microglia morphology, Ca2+ signaling in astrocytes, neurons, pericytes, and endothelia cells, and changes in blood vessel diameter and blood flow.

Hours of usage: 7771 hours

Accessed by: 60 staff/trainees


OUR SERVICES


Carl Zeiss LSM 880 Confocal microscope

Carl Zeiss LSM 880 with AiryScan detector


Bruker Ultima In Vitro two-photon microscope

Bruker Ultima In Vitro


Bruker Ultima In Vivo two-photon microscope

Bruker Ultima In Vivo


Zeiss LCM PALM IV Microdissection microscope

Carl Zeiss PALM MicroBeam


access + Acknowledgement

You can book directly through our Rady Faculty of Health Sciences page on the University of Manitoba website. There you will find all information including consultation, reservations, training, fees, and billing.

We take great pride in assisting you in your scientific endeavors and hope that our services have been helpful in advancing your research goals. We kindly remind you that acknowledging LCIF in your publications is not only a professional courtesy, but also an important factor in securing continued support for our facility. 

Here is a suggestion for acknowledging LCIF in your publications:
โ€œImages presented in this publication were collected and/or processed/analyzed at the Live Cell Imaging Facility at the University of Manitoba.โ€


CONTACT

Noushin Ahmadpour

Imaging Specialist PrairieNeuro Research Center
Kleysen Institute for Advanced Medicine
Department of Pharmacology & Therapeutics
Rady Faculty of Health Sciences
University of Manitoba
SR426, 710 William Avenue
Winnipeg, MB, Canada R3E 0Z3

Dr. Michael F. Jackson

Principal investigator, Facility Director
PrairieNeuro Research Center
Kleysen Institute for Advanced Medicine
Associate Professor
Department of Pharmacology & Therapeutics
Rady Faculty of Health Sciences
SR426, 710 William Avenue
Winnipeg, MB, R3E 0Z3


Publications

Jessica Meza-Resillas, Noushin Ahmadpour, Michael Stobart, Jillian Stobart. Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo. J Vis Exp. 2021 Nov 20;(177). doi: 10.3791/62725. PMID: 34866618.

Chetan S. Patil, Hongbin Li, Natalie E. Lavine, Ruoyang Shi, Ankur Bodalia, Tabrez J. Siddiqui, and Michael F. Jackson. โ€œER-Resident STIM1/2 Couples Ca2+ Entry by NMDA Receptors to Pannexin-1 Activation.โ€ Proceedings of the National Academy of Sciences of the United States of America 119, no. 36 (September 6, 2022): e2112870119. doi: 10.1073/pnas.2112870119.

Noushin Ahmadpour*, Meher Kantroo*, Michael J. Stobart, et al. Cortical astrocyte N-methyl-D-aspartate receptors influence whisker barrel activity and sensory discrimination in mice. Nat Commun. 2024 Feb 21;15(1):1571. doi: 10.1038/s41467-024-45989-3.

Jessica Meza-Resillas, Finnegan Oโ€™Hara, Syed Kaushik, Michael Stobart, Noushin Ahmadpour, Meher Kantroo, Shahin Shabanipour, John Del Rosario, Megan C. Rodriguez, Dmytro Koval, Chaim Glรผck, Bruno Weber, Jillian Stobart. L-Type Ca2+ channels and TRPC3 channels shape brain pericyte Ca2+ signaling and hemodynamics throughout the arteriole to capillary network in vivo. bioRxiv. doi: https://doi.org/10.1101/2024.02.27.582351


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Supported by:

Rady Faculty of health sciences 
Max Rady College of Medicine
Department of Pharmacology and Therapeutics

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